He was the son of Geoffrey Turville, of Towerbank, All Hallows, Barking, London, and Mary Blakney or Blakeney (d. 1642) of Sparham, Norfolk.
[1] The Turvile family estates were at Thurlaston, Leicestershire, however Ambrose's father was a younger son working at the Tower of London.
His sister Ann Turvile married Sir John Leeke of Edmonton.
His mother, now after her third marriage Lady Mary Verney, joined him as a gentlewoman in the queen's privy chamber.
The letter mentioned his half-brother, Sir Francis Verney, who had become a Barbary corsair and was said to have converted to Islam.