It is located in the 422 Business Center in the community of Oaks, Pennsylvania, west of Valley Forge National Historical Park just off of U.S. Route 422.

The collection includes (but is not restricted to): carousel band organs, calliopes, nickelodeons (also known as orchestrions), movie posters and celebrity photographs, vinyl record albums (33-1/3, 48 and 78 speed), Edison cylinder players, antique and modern animated store displays, circus and clown art, pedal cars, model airplanes, and classic cars.

Highlights among them include: The Toy Box takes up the majority of the space in the American Treasure Tour Museum.

Visitors are driven in an electric tram down lanes displayed with an eclectic collection of Americana on both sides and hanging from the ceiling.

Tram tours are conducted with an audio track describing the items, including: automatons, model airplanes, classic cars, more automatic musical instruments, original artwork, movie memorabilia and Disneyana, stuffed animals, animated store displays, motorcycles, holiday displays and neon signage.