Amphicteinae is a subfamily of polychaete worms in the family Ampharetidae.

[1][2] It was originally established as a tribe, with eight genera, but was elevated to subfamily-level with a reduced number of genera in 2020.

[2][3] Two species, Hypaniola kowatewskii and Hypania invalida, are known to feed on blue-green algae.

[4] The subfamily comprises the following genera:[1]