Wat Bot (Thai: วัดโบสถ์, pronounced [wát bòːt]) is a district (amphoe) in the northern part of Phitsanulok province, central Thailand.

Neighboring districts are (from the east clockwise), Chat Trakan, Wang Thong, Mueang Phitsanulok, and Phrom Phiram of Phitsanulok Province; Phichai and Thong Saen Khan of Uttaradit province.

Wat Bot lies within the Nan Basin, which is part of the Chao Phraya Watershed.

Among the areas to be irrigated by the dam's reservoir are Tambon Thap Yai Chiang of Phrom Phiram district, and the Thothae and Tha Ngam Subdistricts of Wat Bot.

[citation needed] Wat Bot is a district on the outskirts of Phitsanulok province, most of the area is in the reserved forest.