In molecular biology, an amplicon is a piece of DNA or RNA that is the source and/or product of amplification or replication events.
In this context, amplification refers to the production of one or more copies of a genetic fragment or target sequence, specifically the amplicon.
Examples include the Amplicor HIV-1 Monitor Assay (RT-PCR), which has the capacity to recognize HIV in plasma; the HIV-1 QT (NASBA), which is used to measure plasma viral load by amplifying a segment of the HIV RNA; and transcription mediated amplification, which employs a hybridization protection assay to distinguish Chlamydia trachomatis infections.
After quality control classification is done by different methods, the counts of identical taxa representing their relative abundance in the sample.
[16] The loci of Alu element insertion is selected, amplified and evaluated in terms of size of the fragment.
The kit adapted for carrying out the method includes a pair of primers to amplify the locus and optionally polymerase chain reaction reagents.
The primers bind adjacent to one another, forming a segment of double stranded DNA that once separated, can serve as a target for future rounds of replication.