Amplicon

In molecular biology, an amplicon is a piece of DNA or RNA that is the source and/or product of amplification or replication events.

In this context, amplification refers to the production of one or more copies of a genetic fragment or target sequence, specifically the amplicon.

Examples include the Amplicor HIV-1 Monitor Assay (RT-PCR), which has the capacity to recognize HIV in plasma; the HIV-1 QT (NASBA), which is used to measure plasma viral load by amplifying a segment of the HIV RNA; and transcription mediated amplification, which employs a hybridization protection assay to distinguish Chlamydia trachomatis infections.

After quality control classification is done by different methods, the counts of identical taxa representing their relative abundance in the sample.

[16] The loci of Alu element insertion is selected, amplified and evaluated in terms of size of the fragment.

The kit adapted for carrying out the method includes a pair of primers to amplify the locus and optionally polymerase chain reaction reagents.

The primers bind adjacent to one another, forming a segment of double stranded DNA that once separated, can serve as a target for future rounds of replication.