Transcription-mediated amplification

Transcription-mediated amplification (TMA) is an isothermal (performed at constant temperature), single-tube nucleic acid amplification system utilizing two enzymes, RNA polymerase and reverse transcriptase.

"Amplification" means creating many more copies of a strand of nucleic acid than was present at first, in order to readily detect it or test it.

Rapidly amplifying the target RNA/DNA allows a lab to simultaneously detect multiple pathogenic organisms in a single tube.

TMA technology allows a clinical laboratory to perform nucleic acid test (NAT) assays for blood screening with fewer steps, less processing time, and faster results.

It is used in molecular biology, forensics, and medicine for the rapid identification and diagnosis of pathogenic organisms.