The Lincoln Service and Missouri River Runner operate separately except for one daily round trip (trains 318 and 319) in which they are combined.

[2] There are 22 Amtrak Midwest round trips each weekday, with the Hiawatha running slightly different frequencies on Fridays, Saturdays, and Sundays.

State-supported service in the Midwest is supplemented by a number of long-distance Amtrak routes, such as the Empire Builder, California Zephyr and City of New Orleans.

[5][6] The Amtrak Midwest logo and brand were unveiled in 2017 alongside the debut of Siemens Charger locomotives on the Hiawatha.

In December 2023, the Federal Railroad Administration accepted a number of Midwest routes into its Corridor Identification and Development Program.