She assisted her husband in his practice, and the couple actively participated in Slovene cultural life as promotors of Pan-Slavism.

All siblings obtained higher education, most notably Ana's sister Eleonora who went on to become the first Slovene female physician.

[3] On 22 July 1911, she received her doctorate from the Philosophical Faculty of the Carl-Ferdinand University in Prague (Czech branch) after major rigorosums in chemistry and physics, and minor in philosophy, with the thesis Studie o stanovení a dělení kyselin: jantarové, jablečné a vinné.

She was not signed as a co-author of the paper he published in 1914 in the journal Zeitschrift für Electrochemie und Angewandte Physikalische Chemie, as was common practice at the time, but her scientific contribution is attested in the acknowledgements section.

Ana Štěrba-Böhm died of cancer on 22 July 1936 in Prague, and is buried at the Vinohrady Cemetery there.