Ana, Princess of Melito and Duchess of Pastrana, married Rui Gomes da Silva, 1st Prince of Éboli when she was 13 years old (1553), by recommendation of the regent of Spain, the future King Philip II.

[3] Ana, Princess of Éboli, had ten children by her marriage:[4] After her husband's death in 1573, she spent three years in a convent, but returned to public life in 1576, forming an alliance at Court with the King's undersecretary of state, Antonio Pérez (1540–1615).

[6] There is a character called Princess Eboli based on Ana in Schiller's play Don Carlos, Infant von Spanien, and Verdi's opera Don Carlos.

The 5th and 6th episodes of 'Teresa de Jesus' filmed for Spanish television in 1984, starring Concha Velasco as Teresa and Patricia Adriani as Eboli, detail the founding of a convent of Carmelite nuns at the princess' request and all of the problems faced.

'La Tuerta', a stage play charting the life of Ana de Mendoza, was performed at Bedlam Theatre as part of The Edinburgh Fringe Festival in August 2008.