In 1939 Sharpenak organized a biochemical department at the Moscow Stomatological Institute (now Moscow State University of Medicine and Dentistry).
Sharpenak, studies the work of a blood enzyme on himself, depending on the diet.While working at the Institute of Nutrition, Sharpenak, who studied protein metabolism, has contributed to the study of some amino acids and their optimum balance.
[5][6] Sharpenak pointed out that the diet should take into account not only the total protein content, but also the content of individual amino acids.
The effect of protein quality of the diet on the body depends not only on the absolute content of individual amino acids in the diet, but also on the balance between them.
[7] In 1949, after numerous experiments, Sharpenak proposed that dental caries was due to insufficient supply in the organs and tissues protein of B vitamins, and an excess of carbohydrates.