For many years, he was an official painter to the Grand Duchess of Tuscany, Vittoria della Rovere.
[1] He often worked in collaboration with Bartolomeo Bimbi, under command of the Médicis, to produce tableaux featuring animals and plants.
His paintings of bouquets are distinguished from those of Bimbi by darker shading, brighter colors and the use of Baroque vases.
[1] There is some indication, from 1698, that he also worked with the sculptor, Giovanni Battista Foggini, making colored mosaic tiles for furniture and other small items.
[2] His son, Pietro Neri Scacciati (1684-1749), was also a painter and appears to have specialized in birds.