Ann Barclay (born January 31, 1940) is a Canadian former tennis player.

Barclay, younger sister of tennis player Lawrence, is a native of Vancouver, British Columbia.

She was part of the inaugural edition of the Federation Cup in 1963, representing Canada in a tie against Great Britain at Queen's Club.

While in England she also competed in the Wimbledon Championships for the only time.

[2] She was a 1994 inductee into the Canadian Tennis Hall of Fame.