Anna of Mecklenburg (14 October 1533 – 4 July 1602), was the duchess consort of Courland by marriage to Gotthard Kettler.

Anna was raised in the Lutheran faith in Mecklenburg, where the reformation had recently been introduced.

She married the Duke of Courland, Gotthard Kettler, on 11 March 1566 in Königsberg.

Anna of Mecklenburg was described as a well educated cultural patron, who is credited for having played an important role in the development of art in the new state of Courland.

She corresponded with her relatives in Germany, maintaining foreign diplomatic connections to the Duchy.