She was the daughter of John Hamilton, 1st Earl of Ruglen (c. 1664–1744),[2] and his wife Lady Anne Kennedy.
[3] The title Earl of Ruglen in the Peerage of Scotland, along with the subsidiary titles Viscount of Riccartoun and Lord Hillhouse, were created on the 14th of April, 1697, for her father Lord John Douglas-Hamilton, fourth (third surviving) son of William Douglas-Hamilton, Duke of Hamilton, 1st Earl of Selkirk, and his wife Anne Hamilton, 3rd Duchess of Hamilton.
As her only brother, William, Lord Daer, died in 1742, upon her father's death in 1744, Anne succeeded to the title Countess of Ruglen suo jure.
[3] She married William Douglas (c. 1696–1731), who inherited the earldom of March in 1705.
Their son:[3] Shortly before her own death, she remarried, her second husband being Anthony Sawyer, Esq, who held the position of Paymaster to the King's forces in Scotland.