It is located on the Argyle Line, 3+3⁄4 miles (6.0 km) west of Glasgow Central (Low Level), on the North Clyde Line 4+1⁄4 miles (6.8 km) west of Glasgow Queen Street (Low Level), and is the terminus of the Maryhill Line 6+1⁄4 miles (10.1 km) away from Glasgow Queen Street (High Level).

The chord from Maryhill (which was part of the original Stobcross Railway route) remains diesel worked.

[3] This was done to provide a diversionary route from the main Edinburgh to Glasgow line into Glasgow Queen Street Low Level while the High Level station was shut during 2016 for tunnel works; however, it is intended that the new connection be permanent.

[6] Destinations that are accessible from Anniesland are Balloch, Milngavie and Dalmuir (Mondays-Saturdays) and Helensburgh Central (Sundays) northwestbound and Whifflet, Motherwell, and Cumbernauld on the Argyle Line and Airdrie (Monday-Saturday daytimes) and Edinburgh Waverley on the North Clyde Line southeastbound.

[7] Since a timetable revision on 18 May 2014, a limited hourly Sunday service operates on the route via Maryhill.