It is the main altarpiece of the first chapel on the right in the church of San Carlo ai Catinari in Rome, Italy.

The Annunciation painting depicts the archangel Gabriel, aloft on a cloud, revealing to the Virgin Mary that she was pregnant with Jesus (Luke 1:10–20 King James Bible).

Above, a group of cherubs open the heavens to allow for light to fall on the Virgin, and to allow the descent (in the form of a dove) of the Holy Spirit.

There is a second, smaller version of The Annunciation by Lanfranco, now displayed at the Hermitage Museum in Saint Petersburg, Russia.

[2] Finally another Annunciation canvas (1636) attributed to Lanfranco is displayed in the church of the Agustinas Recoletas in Salamanca.