The parish of Tarxien was created on May 29, 1592 by Bishop Tomás Gargallo.

It was recorded that by 1621 the construction was still in progress and that in 1627 works on the architecture was completed.

During the 18th century the church underwent extensive changes in its structure and architecture especially in its facade.

The high altar, which was in use prior to the changes brought by the Second Vatican Council, is made of marble and is the work of Antonio Nikolas of Senglea.

The titular painting depicting the Annunciation is the work of Pietro Gagliardi dating from 1874.