Both sexes have a black abdomen bearing two yellow bands on each tergite.

Females are smaller than males and have pale yellowish pollen brushes on the underside of the abdomen.

[2] In both A. florentinum in southern France and A. manicatum in southern Germany, unlike the majority of solitary bees, a study found that there are more females than males, males are bigger than the females, and the sexes fly at the same times of year.

P. Wirtz and colleagues suggest that this pattern is explained by the prolonged sexual receptivity of the females, along with the males' use of readily defended resources in their territories.

[3] A. florentinum obtained from Iran was found to be a more efficient pollinator of alfalfa in the United States than native species.