After completing his classical studies at the Episcopal Seminary of his native city, he moved to Venice, where he spent most of his life.

[3] He struck up a close friendship with Lorenzo Tiepolo, a powerful Venetian senator, and Giovanni Francesco Loredan, the founder of the Accademia degli Incogniti, of which Lupis became a member.

In 1677 he published La Marchesa d'Hunsleij, overo l'Amazone scozzese ("The Marchioness of Huntly, or the Scottish Amazon"), a romanticised hagiographic biography of Lady Margaret Gordon, mother of the Scottish-born Capuchin friar John Forbes (1570/71–1606), that passed through eighteen editions before his death, and was reprinted as late as 1723.

[4] Lupis is the author of L'eroina veneta (1689), one of the earliest and most important biographies of Elena Cornaro Piscopia, the first woman to be awarded a higher university degree.

[8] Of particular interest are a eulogy of his friend, the painter Evaristo Baschenis, written during the artist's lifetime,[9] and the letters sent to the sculptor Andrea Fantoni (1659-1734).