The highway runs northeast through sparsely populated areas of the Ozark Highlands and crossing Fourche River.

Highway 328 continues easterly, crossing into the flatter terrain of the Mississippi Alluvial Plain before turning due south as a section line road, crossing the Current River, and running to Reyno.

ArDOT estimates the traffic level for a segment of roadway for any average day of the year in these surveys.

[3] Highway 328 begins at an intersection with Highway 211 (Stephens Street) in Success in western Clay County three miles (4.8 km) east of the Current River, and three miles (4.8 km) south of the Missouri border.

[4] As of 2016, the route had an annual average daily traffic (AADT) of 660 vehicles per day (VPD).