The route of 69.31 miles (111.54 km) runs from US 65B/US 79B in Pine Bluff north through Little Rock to US 65B in Conway.

Further northwest, Highway 365 continues parallel to I-530/US 65 to Redfield where the route passes original Dollarway Pavement.

Highway 365 passes Little Rock National Cemetery before intersecting I-30/US 65/US 67/US 167 at a frontage road interchange.

Highway 365 crosses over the Arkansas River into North Little Rock and breaks from US 70 to the west near Dickey-Stephens Park.

Highway 365 passes over the railyard, intersecting Riverfront Drive and becoming Pike Avenue to the north.