One segment of 11.8 miles (19.0 km) runs from U.S. Route 165 (US 165) north of Gillett east to the La Grue Bayou.

A third segment of 7.4 miles (11.9 km) runs from Highway 1B in Marianna east to Lee CR 239 at Bear Creek Lake.

[4] The route begins at Highway 1B in Marianna and is locally known as Martin Luther King Jr. Drive in the town.

Highway 44 heads east, out of the town and enters Mississippi River State Park shortly after, and into the St. Francis National Forest.

The route continues to head southeast, and eventually terminates at Lee CR 239 at Bear Creek Lake.