It serves the northern suburb of Ascot Vale in Melbourne, Victoria, Australia.

It opened on 1 November 1860, with the current station provided in 1871.

[4] Ascot Vale station opened on 1 November 1860, just over a week after the railway line to Essendon opened as part of the private Melbourne and Essendon Railway Company.

[4] The station closed with the line on 1 July 1864, but was reopened on 9 October 1871, under government ownership.

[8] Platform 1: Platform 2: Transit Systems Victoria operates two bus routes via Ascot Vale station, under contract to Public Transport Victoria: Yarra Trams operates one route via Ascot Vale station: