The women's 5000 metres T54 event at the 2016 Summer Paralympics took place at the Rio Olympic Stadium on 14 and 15 September.

The United States took all three medals, while eventual winner Tatyana McFadden broke the Paralympic record during the heat stages.

T53 athletes may have spinal injuries or cerebral palsy and have full use of their arms but have no or limited trunk function.

The heats were completed at 10:00 and 10:17 local time.

The first three athletes in each heat and the next four fastest advanced to the final.