The women's 400 metres competition of the athletics events at the 2019 Pan American Games will take place between the 7 and 8 of August at the 2019 Pan American Games Athletics Stadium.

The defending Pan American Games champion is Kendall Baisden from the United States.

Right out of the blocks, Shericka Jackson was moving ahead of the stagger.

Down the backstretch the center lanes of the track, including Courtney Okolo were moving ahead.

Prior to this competition, the existing world and Pan American Games records were as follows: