Ayn Issa Subdistrict or Ayn Issa Nahiyah (Arabic: ناحية عين عيسى) is a Syrian Nahiyah (Subdistrict) located in Tell Abyad District in Raqqa.

According to the Syria Central Bureau of Statistics (CBS), Ayn Issa Subdistrict had a population of 40,912 in the 2004 census.

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