After impressing for Delhi state team during the U14 nationals, Ayush was selected for the U Dream Football programme in Germany, where he spent almost four years.
[2] He made his I-League professional debut for Indian Arrows on 28 December 2019 at Fatorda Stadium against Churchill Brothers, he started the match and was substituted in 86th minute as they won 1–2.
[6] The following game, Adhikari made his first start for the Blasters in Indian Super League, on 25 November 2021, in a 0–0 draw to NorthEast United FC before being substituted for Puitea.
[10] In April 2022, Ayush was included in the Kerala Blasters' reserve team and was appointed as their captain to participate in the inaugural edition of the Reliance Foundation Development League.
In August 2023, Chennaiyin FC announced the signing of Ayush Adhikari ahead of the 2023-24 Indian Super League season.