[citation needed] During the Thirty Years' War, her father-in-law abdicated in favor of Frederick V in April 1622.

At the end of 1622, the couple fled with their five children to the court of Barbara's brother, John Frederick, Duke of Württemberg, in Stuttgart.

Frederick managed to free his lands the following year, but already in 1624 the troops of the Catholic League invaded the country again and occupied Pforzheim.

[2] She was buried at the castle church of Pforzheim, where there is an epitaph to Barbara of Württemberg engraved in the floor of the choir.

[6][1] In 1909, art historian Ernst Lembert suggested that the painter was a Nuremberg artist named Maria Eißler.