Barletta railway station is situated at Piazza Francesco Conteduca, on the southern edge of the city centre.

[1] The since closed link between the station and Barletta Marittima, mentioned above, was opened on 1 August 1895.

There are other through tracks serving the goods yard, although it is not used much today, especially in light of the closure of the line to Barletta Marittima.

All trains stop at the station, including regional, interregional, express, InterCity and Eurostar.

The station is also the terminus for all secondary line trains to Spinazzola and Bari Centrale (via the FNB), respectively.