Barnaviridae is a family of non-enveloped, positive-strand RNA viruses.

The family has one genus, Barnavirus, which contains one species: Mushroom bacilliform virus.

[1][2] Viruses in Barnaviridae are non-enveloped, with icosahedral and Bacilliform geometries, and T=1 symmetry.

The single capsid protein (ORF4) is translated from a subgenomic RNA.

Cultivated mushroom, Agaricus bisporus, serves as the natural host.