Located on Beallsville Road (Route 109), Barnesville station services trains of MARC Train's Brunswick Line between Union Station in Washington D.C. and Martinsburg, West Virginia.

Amtrak's Capitol Limited operates through the station, but does not make any stops.

Barnesville station contains two low-level side platforms, connected by a pathway across the tracks.

Service in Barnesville began with the opening of the Metropolitan Branch Railroad on May 25, 1873.

This Maryland train station-related article is a stub.