Barra del Colorado Wildlife Refuge is a wildlife refuge, part of the Tortuguero Conservation Area, in Limón Province in the northeastern part of Costa Rica.

The Refuge is bounded in part by the San Juan and Colorado rivers, and is located to the south the Tortuguero National Park.

The area protected has a hot and humid climate with no dry season, and is the habitat of the endangered West Indian manatee as well as many caymans, crocodiles, and fish.

It is also home to a variety of tapirs, jaguars, cougars, monkeys, ocelots, and other mammals.

Birds found include osprey, toucans, cormorants, herons, hawks, and many more.