Base calling is the process of assigning nucleobases to chromatogram peaks, light intensity signals, or electrical current changes resulting from nucleotides passing through a nanopore.
One computer program for accomplishing this job is Phred, which is a widely used base calling software program by both academic and commercial DNA sequencing laboratories because of its high base calling accuracy.
[1] Base callers for Nanopore sequencing use neural networks trained on current signals obtained from accurate sequencing data.
[2] Base calling can be assessed by two metrics, read accuracy and consensus accuracy.
Consensus accuracy refers to how accurate a consensus sequence is compared to overlapping reads from the same genetic locus.