Bernhard V, Prince of Anhalt-Bernburg (died 24 June 1420) was a German prince of the House of Ascania and ruler of the principality of Anhalt-Bernburg.
He was the eldest son of Henry IV, Prince of Anhalt-Bernburg, by his wife Sophie, a possible member of the House of Stolberg.
After the death of his father in 1374, Bernhard was bypassed as heir during the rule of his uncle Otto III.
When Otto died in 1404, Bernhard finally took possession of the principality of Anhalt-Bernburg, but he was compelled to rule jointly with his youngest cousin Otto IV until the latter's death in 1415.
On 8 September 1396, Bernhard married Elisabeth (d. 1426), daughter of Ulrich III, Count of Honstein-Kelbra.