Henry IV, Prince of Anhalt-Bernburg (died 7 July 1374) was a German prince of the House of Ascania and ruler of the principality of Anhalt-Bernburg.
He was the second son of Bernhard III, Prince of Anhalt-Bernburg, by his first wife Agnes, daughter of Rudolf I, Duke of Saxe-Wittenberg.
In addition to his princely title, he also adopted the style "Lord of Bernburg".
Henry married Sophie, possibly a member of the House of Stolberg.
They had three children: After his death, his son Bernhard was bypassed in his rights over Bernburg in favor of Henry's younger half-brother Otto, who succeeded him.