The municipality of Biberstein, probably including the castle, is first mentioned in 1280[1] which indicates a late 13th century construction date.

After 1368 the Habsburgs attempted to secretly repurchase Biberstein Castle from the Knights by using a minor noble as a straw purchaser.

[1] In 1415 the city-state of Bern occupied part of Aargau including Biberstein, though the Knights continued to own and rule over the town and castle.

During the Swabian War of 1499 Bernese troops occupied Biberstein Castle to protect their northern border.

In 1527 Bern adopted the new faith of the Protestant Reformation and began to suppress the monasteries in their lands.

The remaining walls and castle structure generally date to the 16th century reconstruction or later renovation projects.