Botswana athletes achieved the entry standards, either by qualifying time or by world ranking, in the following track and field events (up to a maximum of 3 athletes in each event):[2][3] Botswana entered two boxers into the Olympic tournament for the first time since London 2012.

Keamogetse Kenosi scored an outright semifinal victory to secure a spot in the women's flyweight division at the 2020 African Qualification Tournament in Diamniadio, Senegal.

[4] Rajab Otukile Mahommed completed the nation's boxing lineup by topping the list of eligible boxers from Africa in the men's flyweight division of the IOC's Boxing Task Force Rankings.

Botswana received a universality invitation from FINA to send a top-ranked male swimmer in his respective individual events to the Olympics, based on the FINA Points System of June 28, 2021.

Magdeline Moyengwa topped the list of weightlifters from Africa in the women's 59 kg category based on the IWF Absolute Continental Rankings.