On July 8, 2022, Jenkins was appointed interim district attorney by Mayor London Breed following the successful recall of Chesa Boudin, for which she actively campaigned.

[5] She was raised by her mother because shortly after her birth, her father had to leave the country due to his student visa status.

[5] She resigned in October 2021 to support the recall campaign against San Francisco District Attorney Chesa Boudin.

[18] Proposition E was criticized by organizations like the ACLU, the Electronic Frontier Foundation, the San Francisco Public Defender's Office, among others for privacy concerns and potential for the suppression of dissent.

On October 13, 2022, retired Superior Court Judge Martha Goldin filed a State Bar complaint against Jenkins, outlining multiple misconduct allegations.

[20][21] Jenkins was paid a six-figure consulting fee by the nonprofit Neighbors for a Better San Francisco, which shares a name and an office with the Chesa Boudin recall campaign.

[25] In August 2023, the California Court of Appeal, First District, found that Brooke Jenkins committed prosecutorial misconduct in a homicide case she prosecuted in 2021 by making improper arguments about the defense attorney in violation of ethical rules.

[27] In July of 2024, reporter Jonah Owen Lamb uncovered that Brooke Jenkins hired a UCSF nursing professor and longtime friend from high school, Monifa Willis, with no legal experience to run the San Francisco District Attorney's Office as Chief of Staff, receiving an annual compensation of nearly $300,000 while maintaining outside employment.