Bulked segregant analysis (BSA) is a technique used to identify genetic markers associated with a mutant phenotype.
These two bulked samples can then be analysed using techniques such as Restriction fragment length polymorphism or RAPD to detect similarities and differences in the various loci of the genome.
In animals, the individuals making up the two testing groups are usually produced by a cross between two siblings heterozygous for the mutation of interest.
Use of restriction enzymes or PCR amplification on the DNA is required for RFLP or RAPD analysis respectively.
In these techniques, the loci that are analysed are the restriction digest sites and the sequences on which PCR primers attach to.