Busitema is a settlement in eastern Uganda.

It is the location of the Main Campus of Busitema University, one of the public universities in Uganda.

[1] Busitema is located approximately 23 kilometres (14 mi), by road, northwest of Busia, where the district headquarters are located.

[2] This location is approximately 26 kilometres (16 mi), by road, southwest of Tororo, the largest town in the sub-region.

As of April 2010, the exact population of Busitema is not known.