The Castello di Santa Margherita Ligure is a 16th-century fortress in Santa Margherita Ligure, within the Metropolitan City of Genoa in Italy.

The fortress is located at the foot of a hill on which the Villa Durazzo-Centurione [it] now stands.

It was built following a resolution by the Senate of the Republic of Genoa in 1550, and it was meant to defend against raids from the Barbary pirates.

Various structural interventions and armament upgrades took place until the early 17th century.

After pirate attacks ceased in the 18th century, the fort lost its military importance and only some repair and maintenance works were undertaken.