The trail begins southwest of Smiths Falls, at a parking lot south of Ontario Highway 15 designated as kilometre zero (44°53′24″N 76°01′50″W / 44.89000°N 76.03056°W / 44.89000; -76.03056).

In its midsection the trail crosses the UNESCO Frontenac Arch Biosphere Reserve.

The 78.2-kilometre (48.6 mi) segment running from Smiths Falls to Harrowsmith is part of the Trans Canada Trail.

The Rideau Canal is crossed on a 1912 railway trestle at Chaffey's Locks, near kilometre post 42.

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