The Central Florida Railroad Museum is located at 101 South Boyd Street, Winter Garden, Florida in a former Tavares and Gulf depot built in 1913.

The museum contains exhibits depicting Central Florida's railroading history, including a large collection of dining car china.

[3] It is part of the Winter Garden Downtown Historic District, which is listed on the National Register of Historic Places.

The museum is operated by the Central Florida Railway Historical Society.

The museum operates in conjunction with the Winter Garden Heritage Foundation.