[4] The species is named for Edward Burnham Chamberlain, a former curator of the Vertebrate Zoology department at the Charleston Museum.

[6] E. chamberlaini is known to be a lighter brown color than E. quadridigitata (which it is commonly confused with) and has a yellowish underbelly with no spots.

[10][11] Chamberlain's dwarf salamander can be found in lotic environments and prefers the areas around streams or seepage from springs and ponds.

The reproductive season is October through April[8] with the eggs being laid around winter time and hatching during spring.

[5] The males are considered "searching morphs" meaning they coerce the females using pheromones to mate with them.

[8] Some individuals even metamorphosed in just two months, which suggests that E. chamberlaini has an extremely short larval period.

[4] Hatchlings are a light-grayish brown with well-developed dorsal fins that stretch from the tail to the mid-body area.