The Charles County Sheriff's Office (CCSO) is a nationally accredited, full-service law enforcement agency servicing a population of 166,617 within 457.8 square miles (1,186 km2) of Charles County, Maryland, United States.

[1] The CCSO was created in 1658 with the appointment of Nicholas Gwyther as dual Sheriff of Charles County and Saint Mary's County.

[2] Sheriff Troy Berry retired in October 2022, although he remained on the ballot for the election in November.

The agency employs more than 900 sworn and civilian employees, making the Charles County Sheriff's Office is the largest sheriff's office in the state.

The CCSO is divided into nine divisions and the office of the sheriff.