[2] In her early years in Berlin, Charlaque performed as a singer, dancer and actress, later she also worked as a language teacher and translator as well as a receptionist at Magnus Hirschfeld's Institute for Sexual Science.

In 1929, Charlaque also accompanied Magnus Hirschfeld and his partner Karl Giese to the third international congress of the World League for Sexual Reform (WLSR) in London.

Alongside the kitchen assistant Dora Richter and the painter Toni Ebel, with whom she was friends, she was one of the first three known cases of gender reassignment surgery worldwide.

As Charlotte Charlaque was Jewish, Toni Ebel converted to Judaism in the early 1930s and both women were staunch opponents of National Socialism, they fled together to Czechoslovakia in the spring of 1934, where they first settled in Karlovy Vary and later in Brno and Prague.

While Ebel painted pictures for spa guests and other clients, Charlaque taught English and French, probably also to Jews who were fleeing persecution by the German National Socialists.

[2] A few months before the invasion of Czechoslovakia by the German Wehrmacht on March 15, 1939 and the establishment of the "Protectorate of Bohemia and Moravia", events came to a head for Charlaque and Ebel.

[5] In her private life, she was in contact with the German-American doctor and endocrinologist Harry Benjamin, the "cross-dresser" Louise Lawrence (1912–1976) and Christine Jorgensen, who attracted a great deal of media attention in the course of her gender reassignment in 1952.

At a funeral service a few days later, she was honored in a memorial speech by William Glenesk (1926–2014), who was known as an innovative clergyman and later also as an advocate for people from the LGBTIQ spectrum.