Charlotte of France (23 October 1516 – 18 September 1524) was the second child and second daughter of the French King Francis I and his wife Queen Claude.

Princess Charlotte was born on 23 October 1516 at the Château d'Amboise (in the Loire Valley in France) as the second daughter and child of King Francis I and Queen Claude.

At age seven, she contracted measles, the same disease which had killed her uncle, Charles Orlando, Dauphin of France, thirty years earlier.

The only person who looked after her while she was sick was her aunt, Margaret of Angoulême, as her mother had already died two months earlier, her grandmother Louise of Savoy was very sick and her father was occupied by war.

Charlotte likely was very close to her aunt, who was heartbroken and distraught when her "little one"[citation needed] died.