Languages of publication are Czech and Slovak; English manuscripts can only be submitted upon invitation.

The journal was initially focused on practical and technical chemistry but, after 1882, scientific contributions began to dominate.

In 1922, Jaroslav Heyrovský (Nobel laureate, 1959) first published in Chemické Listy the basic principle of polarography: "Electrolysis With a Dropping Mercury Cathode".

[2] The following persons are or have been editors-in-chief of the journal: The journal is abstracted and indexed in Chemical Abstracts,[3] Current Contents/Physical, Chemical & Earth Sciences,[4] Science Citation Index,[4] and Scopus.

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