After Hagerstown, the Historic National Road Scenic Byway follows US 40 Alternate through Funkstown to Boonsboro, where Washington Monument State Park is located.

The Mountain Maryland Scenic Byway followed US 219 to McHenry, which is home to Wisp Ski Resort and Deep Creek Lake State Park.

The Chesapeake and Ohio Canal Scenic Byway heads south on MD 109 into Poolesville, where the John Poole House and the Seneca Schoolhouse is located.

Another branch off the byway runs through areas protected by the Montgomery County, Maryland Agricultural Reserve, with the road continuing along MD 118 to Germantown, a small town with antique shops.

From here, the byway continues north on MD 109, passing through Beallsville and Barnesville before reaching Comus, where Lee's army won a rearguard action.

[1] The Old Main Streets Scenic Byway loops through small rural towns across the northern part of Maryland between Emmitsburg and Mount Airy.

The Old Main Streets Scenic Byway continues east on MD 77, passing through the community of Detour, which developed along the Western Maryland Railway.

From here, the Mason and Dixon Scenic Byway continues along White Hall Road, turning north onto MD 23 and passing through Drybranch and Norrisville.

Here, the byway heads east on MD 136 and runs a short distance south of the Mason–Dixon line separating Maryland from Pennsylvania, reaching Harkins.

[1] In Harkins, a loop of the Mason and Dixon Scenic Byway heads south on MD 24, passing Eden Mill Nature Center and Rocks State Park.

[1] From Harkins, the mainline of the Mason and Dixon Scenic Byway continues east along MD 136, passing through Whiteford, where there are many agricultural sites nearby.

The road comes to the Fair Hill Natural Resource Management Area, which occupies former land owned by the Du Pont family and has equestrian facilities, before the byway ends at the Delaware border in Appleton.

[1] From Glen Arm the Horses and Hounds Scenic Byway has a long sidetrack that heads east toward Jerusalem, which contains one of several units of Gunpowder Falls State Park.

A spur of the byway continues northwest to MD 161 to pass through the village; the main route turns northeast onto Shuresville Road toward Conowingo Dam.

The byway runs through Belvedere Row before reaching Druid Hill Park area, which is home to the Baltimore Museum of Art and the Maryland Zoo.

Charles Street heads into Baltimore County, where MD 134 provides access to Lutherville and a spur serves Towson, a major commercial center.

From Fells Point, the National Historic Seaport Scenic Byway continues west on Fleet Street to the Little Italy neighborhood, which has many Italian restaurants and shops.

The byway follows Sixes Road to MD 231, reaching Benedict, a landing point for British troops heading inland along the Patuxent River in August 1814.

The mainline of the byway continues along MD 234 across Zekiah Swamp before coming to Chaptico, a port settlement where Christ Episcopal Church is located.

The Religious Freedom Byway continues to Point Lookout State Park, which was a summer resort that also served as a prison for Confederate troops during the American Civil War.

A state-designated extension of the national byway continues across the Bay Bridge, into Annapolis, then back onto the Eastern Shore and heads south toward Crisfield.

Both the national and state-designated sections of the byway run past many towns and buildings that remain preserved from the 18th and 19th centuries, passing through farmland and waterfront areas on the Chesapeake Bay.

[1] From Centreville, the Mid-Shore Section of the Chesapeake Country Scenic Byway continues on MD 213, with a spur along US 50 providing access to the Wye Mills Natural Resource Management Area.

The byway continues along MD 662 before picking up US 50 and coming to Easton, a historical town which is home to the Avalon Theatre and the Academy Art Museum.

The byway continues to Preston, following MD 16 south to East New Market, a Native American trading post that became a center of Methodism.

At this point, a branch of the byway heads south along MD 341 and Maple Dam Road into the Blackwater National Wildlife Refuge, which is home to the largest amount of bald eagles on the East Coast.

Another branch of the byway continues south along MD 335, passing to the west of the Blackwater National Wildlife Refuge before reaching Hooper's Island.

The Chesapeake County Scenic Byway follows MD 362 east to Princess Anne, a historic town founded in 1733 that is home to the Teackle Mansion.

From Preston, the route continues along MD 16 to Potters Landing, a crossing point for slaves on the Underground Railroad, and Martinak State Park.

The byway continues along MD 313 to Denton, where several sites related to the Underground Railroad are located including Courthouse Square, the Tuckahoe Neck Meeting House, and the Museum of Rural Life.