Chiesa Madre or "Mother Church" of Carlentini is a baroque-style, Roman Catholic church located on via Via Roma #140, corner with via Garibaldi, in center of the town of Carlentini in the province of Syracuse, region of Sicily, Italy.

A church at this site was begun with the foundation of the town in 1551, under the patronage of the viceroy Giovanni De Vega, and completed by 1609.

However, that structure was virtually razed by the 1693 Sicily earthquake, and reconstruction proceeded slowly over the next centuries.

It is decorated with the coat of arms of Charles V, Holy Roman Emperor, for whom the town was named.

The central nave ceiling was frescoed with a representation of the Immaculate Conception with the Holy Trinity and St Michael Archangel.